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The methods for generating a cpDNA consensus sequence, sequencing editing, and alignment follow those employed for the nrDNA cistron (Fig. 2).
SomaticSniper uses a Bayesian probability calculation to estimate the posterior probability of each possible joint genotype across the normal and cancer samples given the data observed and prior genotype likelihoods based on the reference sequence, sequencing error rate, population mutation rate and somatic mutation rate.
YJ and CG assisted with sequencing, sequence analysis and annotation.
SJP carried out DNA sequencing, sequence assembly, sequence analysis, other molecular genetic analyses and co-wrote the manuscript.
GM, AC, BSand SSwere implicated in BAC clone sequencing, sequence assembly and verification of assembled sequences.
KF, GT and LP performed PCRs, KF and LP did all sequencing, sequence analysis and annotation.
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Table 2 Optimal background subtraction parameters for various sequences Sequence number Optimal n Optimal s Optimal Th 1 19 10.015 3 19 15 0.005 4 18 1414 0.01 5 14 13 0.015 8 8 8 0.01 9 18 5 0.015 Table 3 Area of the smallest ground truth object in each sequence Sequence Object area 1 458 3 1047 4 696 5 677 8 70 9 1468.
Here we present results focused on Parts and the description of their nucleotide sequence, Sequence Features.
Sequence data included the HIV-1 nucleotide sequence, sequence accession number, sequence genotype information, and sequence length.
Target sequence: sequence of the target gene from the respective study.
In the absence of a barley genome sequence, sequence information is restricted to EST databases.
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