AFL participated in gene sequencing, sequence alignment, association test analysis and in manuscript preparation.
HMB carried out nematode extraction and sequencing, sequence alignment, tree building, and drafted the manuscript.
IC carried out the molecular genetics studies, sequencing, sequence alignment, statistical analysis, and drafted the manuscript.
PVB carried out cloning, sequencing, sequence alignment and drafting of the manuscript.
Despite these advantages, this technique requires post-PCR processing, DNA sequencing, sequence alignment, and sequence analysis with the program BlastN or the construction of phylogenetic trees.
For a genome with large number of repetitive sequences, sequence alignment errors can be generated if sequence alignment is totally relied on computer software [ 4].
Thus, the LccED is the first data resource that combines information on sequences, sequence alignments, annotations, and structures of MCOs.
It defines exchange formats for the most common data types: biological sequences, sequence alignments, sequence annotation and references to data, resources and vocabularies.
Sequences encoding RNase P RNAs of various genomes have been determined and a database established [ 53], which consists of a compilation of ribonuclease P RNA sequences, sequence alignments, secondary structures and three-dimensional models.
These methods can be classified into two categories: sequence-sequence alignment methods and sequence-structure alignment methods [ 13].
Align2d algorithm of the MODELLER program is different from standard sequence-sequence alignment methods because it takes into account structural information from the template when constructing an alignment.
