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After ethanol precipitation, automated sequence separation and detection was done on an ABI 3730XL sequencer.
Equivalent work requirements of base direct sequence separation system was 5.59 MW.
Certainly, the latest frontiers in micro and nano-technology which allows high resolution protein sequence separation as well as Mass Spectrometry imaging that can resolve drug deposition that are localized in tumor environments.
Studies of circular permutants have demonstrated that the folding reaction of S6 from Thermus thermophilus (S6T) is malleable and responds in an ordered manner to changes of the sequence separation between interacting residues: the S6T permutants retain a common nucleation pattern in the form of a two-strand-helix motif that can be recruited from different parts of the structure.
Hence contact vectors have a distinct biophysical meaning: they estimate the number of backbone hydrogen bonds ordered by sequence separation.
This is perhaps due to the significance of secondary structural relationships in this range of primary sequence separation.
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It has been further suggested that short sequence separations may be preferred for the establishment of stable interactions for the design of novel artificial enzymes and the modification of slow-folding proteins with aggregating intermediates.
A customary practice is to express sequence separations as distances, which play a role in the construction of trees.
A large-scale analysis showed that there exists a natural cut-off for k, and contacts with longer sequence separations contributed significantly less [23].
Briefly, the large background for short sequence separations (the black 'diagonals' in presented maps) prevented use of MOHCA-seq constraints for short sequence separations.
The frequency is also suppressed by signal attenuation for long sequence separations j − i due to the possibility of reverse transcription termination between i and j.
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