Sentence examples for sequence reverse from inspiring English sources

The allele-specific reverse primer includes both the flanking sequence reverse primer and the sequencer-specific reverse priming site.

The cDNA can then be directly sequenced (after library adapter ligation) and as each read will contain multiple copies (typically three) of the same viral RNA sequence, reverse transcriptase errors and sequencer base miscalls can be readily identified; a similar strategy to that of utilising overlapping read pairs [ 9].

Importantly, this method retains the ability to sequence reverse transcription polymerase chain reaction (RT PCR) products, validating microRNA-specific amplification.

Then, using an oligodT primer harboring a consensus sequence, reverse transcription was performed on total RNA using SuperScript II RT (Invitrogen).

In regions of low quality sequence, reverse read primers were manually picked.

This information includes UniSTS ID, GenBank accession number, forward primer sequence, reverse primer sequence, and primer purchasing and inventory information.

The main challenge was the novel's convoluted structure: the chapters are ordered chronologically until the middle of the book, at which point the sequence reverses; the book thus begins and ends in the nineteenth century.

Similarly, OPN-B overexpression significantly reduced apoptosis under hypoxia and serum starvation in HT1080 cells, and deletion of the RGD sequence reversed this effect (Fig. S2).

To confirm the nbs1 gene structure and sequence as predicted by the Broad Institute genome sequence, reverse-transcription (RT) PCR was used on purified poly-A RNA from a J6 5x4 K+6 mushroom (SuperScript One-Step RT-PCR for Long Templates, Invitrogen; PolyATtract mRNA Isolation System III, Promega).

Because the enantioselectivity is exclusively determined in the first step of the tandem sequence, reversing the order of the α-iminoester addition gives the opposite enantiomer (Scheme 12).

The surface of the chip was then regenerated using 25 mM NaOH and the injection process was repeated, but with the antibody injection sequence reversed.