Zeng, Y. & Cullen, B. R. Sequence requirements for micro RNA processing and function in human cells.
Amino acid sequence requirements for super stable complex formation with its ligand.
Benoist, C. & Chambon, P. In vivo sequence requirements of the SV40 early promotor region.
Minimal sequence requirements for binding were first established using both crystallography and peptide spot synthesis.
Gregorieff, A., Cloutier, J.F. & Veillette, A. Sequence requirements for association of protein-tyrosine phosphatase PEP with the Src homology 3 domain of inhibitory tyrosine protein kinase p50 (csk).
Schulze, A., Schieck, A., Ni, Y., Mier, W. & Urban, S. Fine mapping of pre-S sequence requirements for hepatitis B virus large envelope protein-mediated receptor interaction.
Sarma, K., Levasseur, P., Aristarkhov, A. & Lee, J. T. Locked nucleic acids (LNAs) reveal sequence requirements and kinetics of Xist RNA localization to the X chromosome.
Egloff, M. P. et al. Structural and functional analysis of methylation and 5′-RNA sequence requirements of short capped RNAs by the methyltransferase domain of dengue virus NS5.
Once students decide to pursue the Biological Chemistry track, they should adhere to the alternative sequence requirements (e.g., complete Chem 12B, not Chem 3B).
This though has become less of an issue as additional CRISPR endonuclease variants with different specificities have been identified from other microbes and as new PAM sequence requirements have been engineered into Cas9.
We probed in detail the sequence requirements of a small single-stranded domain using a combination of in silico, in vitro and in vivo virus experiments.
