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Lobanenkov et al. suggested that additional DNA flanking outside the CTCF recognition motifs are required for tight binding but the exact sequence requirement for this flanking DNA may not be as strict as that of the CCCTC motifs (Lobanenkov et al., 1990).
Also, the lack of a strict sequence requirement for the extra-ribosomal section seems to disfavor the allosteric transmission theory.
Koike, M. β-catenin shows an overlapping sequence requirement but distinct molecular interactions for its bidirectional passage through nuclear pores.
Next, to ascertain if there is a specific 5′-extension sequence requirement for this enhancement, three different 59 nucleotide 5′ extensions were compared.
This suggests that there is not a stringent sequence requirement for the 5′-extension enhancement, similar to previous findings with the 9 nucleotide extended crRNAs with electroporated cells (Fig. 2).
This sequence requirement contributes to the target selectivity of the compound, and thus to its efficacy and relatively limited toxicity [20].
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Benoist, C. & Chambon, P. In vivo sequence requirements of the SV40 early promotor region.
Minimal sequence requirements for binding were first established using both crystallography and peptide spot synthesis.
Egloff, M. P. et al. Structural and functional analysis of methylation and 5′-RNA sequence requirements of short capped RNAs by the methyltransferase domain of dengue virus NS5.
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