Sentence examples for sequence replacement from inspiring English sources

If necessary, this step may involve sequence replacement in order to match the (same size) sequences in the template library.

This technology utilizes easily reprogrammable guide RNAs (sgRNAs) to direct Streptococcus pyogenes Cas9 endonuclease to generate DNA double-stranded breaks in targeted genome sequences, which facilitates efficient mutagenesis by error-prone nonhomologous end-joining (NHEJ) or sequence replacement by homology-directed repair (HDR).

Within the intracellular region of LRP6, the miniC region of LRP6, especially the PPPSPxS motif, might be the GSK3β inhibitory sequence; replacement of the cytoplasmic domain of LRP6 with this region can induce Wnt/β-catenin signaling even in the absence of the LRP6 extracellular region [12], [13].

The apparent absence or rarity of sequence replacement may indicate a loss of ability for homologous recombination, even though the genes for natural competence are present in A. baumannii [40] and strains of A. baumannii clearly are able to incorporate foreign DNA.

A conditional knockout allele for Lpcat3 was generated with a sequence replacement 'knock-out first/conditional-ready' gene-targeting vector.

However, the origins of this difference in incorporation rate remain unclear given the apparent equivalence of the two protein sequences seen after sequence replacement.

By providing recombinogenic ends for exonucleases or helicases to further act on (DuBose et al. 1988) and limiting the size of transferred fragments, such systems could facilitate small and multiple sequence replacements (McKane and Milkman 1995).

Sequence-replacement mutants were generated either by ligation of a double-stranded oligonucleotide sequence into a linearized construct lacking the sequence to be replaced or by performing PCR with primers that contained sequence corresponding to the replacement sequence at their 5′-end (see Supplementary Figure S1 at http://www.biochemj.org/bj/456/bj4560139add.htm).htm

Proposed sequence of replacement of states for peak sampling and through sampling is defined in such a way that distortion of the waveform be null or minimum possible.

Double crossover homologous recombination of pDSM387 with the WT chromosome results in the complete deletion of the fur (HP1027) coding sequence and replacement with the upstream fur-kan-sacB-downstream fur product.

Both repeats were necessary for RaaS binding, as truncation of the sequence or replacement of three thymidines in one repeat with adenosines led to complete loss of binding (Fig. 4C).