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For example, two of the sixteen endpoint lineages presented exactly the same genetic sequence, rendering it impossible to distinguish them, regardless of the method employed.
Importantly, the Swi6 and EGFP moieties were separated by a GDGAGLIN linker sequence, rendering the Swi6-EGFP fusion protein fully functional (Keller et al, 2013).
PCR methods target and amplify a specific DNA sequence, rendering low levels of target DNA detectable and quantifiable by means of fluorescence.
However, of those that are located within coding sequence, most are non-synonymous and are not associated with the alteration of the amino acid sequence rendering them of no functional consequence.
Although the hoxb7a gene of Astatotilapia burtoni was identified and annotated without problems, the coding sequence contains a stop codon at the beginning of the coding sequence, rendering it a pseudogene.
The fact that this self-matching spacer is tolerated in this organism implies that either the proto-spacer associated motif (PAM) has been mutated in the chromosomal gene sequence rendering the gene immune [ 3] or that the chromosomal CRISPR/Cas system had become deactivated in this archaeon.
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The latest installment — in which the animals Alex, Marty, Gloria and Melman join a circus on the Continent — benefits from a nice supporting cast (Bryan Cranston, Jessica Chastain), clever 3-D effects and a dazzling Cirque du Soleil-style big-top sequence rendered with a pastel palette.
Point mutations in the DrAqp3b amino acid sequence rendered two constructs, DrAqp3b-T85A shigher higher water and ethylene glycol permeability at neutral and alkaline pH, and DrAqp3b-H53A/G54H/T85A, no longer inhibited at acidic pH but less permeable than the wild type.
An EMS-induced point mutation of Avra10, producing premature termination of the coding sequence, rendered the pathogen virulent to plants carrying the Mla10 resistance gene (i.e. avirulence was lost, so the Mla10 plant did not detect the fungus and induce effective defences) [ 4].
These technologies include tools and methods for sequence analysis (nucleotide and protein sequences), rendering of secondary and tertiary structures for these molecules, and protein fold prediction that can lead to rational drug design.
RDP sequences rendering best scoring BLAST hits to the pyrosequencing reads, as well as E. coli sequence S000380829, were downloaded in pre-aligned format from RDP and imported into ARB[33].
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