Exact(3)
One of the advantages of transcriptome pyrosequencing is in terms of sequence reliability.
Rambaut, University of Oxford), while Geneious (Biomatters Ltd., Auckland, New Zealand) and Sequencher v4.8 (Genes Code Corporation, Ann Arbor, MI) were used to check coverage and sequence reliability.
After screening for contaminants using NCBI BLAST [ 65], the high quality forward and reverse sequences of each gene from each sample were aligned and a consensus sequence was inferred using a phred score of 30, i.e. a 99.9% accuracy of a base-call, as the detection threshold, resulting in an increase in sequence lengths and an improvement in sequence reliability.
Similar(57)
The quality tags of a gametophyte clone were aligned into RAD-tag piles with those covering only one tag discarded in order to ensure sequencing reliability.
To verify sequencing reliability, the short read data set was aligned to the Chinese hamster mitochondrial genome (NC_007936.1) and resulted in significant homology.
In addition, to confirm sequencing reliability, we compared the result of direct-sequencing at the polymorphic sites in uncloned PCR product with cloned sequences (see Additional File 2: Figure S2).
Sequence alignment reliability was tested using the HoT procedure (Landan and Graur 2007), and only alignments with a sum-of-pairs score >80% were included in the analysis.
Analysis and multiple alignments of sequences were performed with DNAMAN Version 6.0 and graphic data were analyzed to check sequencing results reliability with Chromas Version 1.4.5 and FinchTV version 1.4.0.
With more input sequences, the reliability of the outer decoding can be enhanced.
The OSD decoding resumes by reordering the received sequence of reliabilities ri, i=1,2,…,N.
Had the exercise performance of each participant been recorded and the examiners allowed to see the video multiple times as well as having the option of slowing and freeze framing the video sequences the reliability scores might have been higher.
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