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The obtained sequence readings were assembled and processed using either DNASTAR® (DNASTAR, Inc., USA) or Vector NTI Advanced version 10.3.0 (Invitrogen. Co., USA).
Shotgun sequence readings of the silkworm Genome (GenBank: BAAB01161029, AADK01014604) covered partial of the Bm-yellow cDNA.
Shotgun sequence readings of the silkworm Genome (GenBank: AADK01003428, AADK01027742) covered partial of the cDNA of Bm-yellow-f2.
Shotgun sequence readings of the silkworm Genome (GenBank: BAAB01152328, AADK01044238) covered the whole cDNA of Bm-yellow-b and no intron was identified in the genomic locus of Bm-yellow-b.
Shotgun sequence readings of the silkworm Genome (GenBank: BAAB01124731, AADK01025556, BAAB01064927, AADK01021354, BAAB01083903, AADK01026184) covered partial of the Bm-yellow-c cDNA, which enabled us to conclude at least 9 introns were contained in the genomic locus of Bm-yellow-c.
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Two contigs were assembled by Phred/Phrap [ 59, 60] from the shotgun sequencing readings, guaranteed to have 10 times coverage for both contigs.
Sequencing is realized on the purified products using the ABI PRISM Big Dye Terminator TM cycle sequencing Reading Reaction Kit (Applied Biosystem).
The Royal Court has embarked upon a scintillating sequence of readings in honour of the playwright Caryl Churchill's 70th birthday.
In a revelatory sequence of readings titled "Lincoln and Booth: Shakespearean Dreams," Alec Baldwin and Annette Bening performed Act III, Scene 2 of "Macbeth" — a Lincoln obsession.
It has two parts: (1) the sequence of readings, including close attention to the methods and evidence on which they draw, and (2) a field practicum in which students will observe language in an actual social setting, and produce field notes guided by questions that we will discuss at length.
Family 1 offspring had marginally better average sequence quality readings than family 2, but the number of RAD alleles defined, the number of RAD alleles per locus, and the number of SNPs were all reasonably consistent between the two families.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com