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The sequence is proof of Ms. Ramsay's bewitching eye and imagination, and "Ratcatcher" is the most lovely debut in a long time.
The sequence is proof of Ms. Ramsay's bewitching eye and imagination, and "Ratcatcher" is the most lovely debut film in a long time.
Since the validity of Theorem 3.1 iii) is independent of the modification of Condition 1 to the weaker one Condition 2 implying the use of the sequence (see proof of Corollary 3.5), Condition 2 of Corollary 3.5 may be replaced with the following.
Positive on direct sequencing means: proof of a mutation.
Sequences were proof read and assembled using SeqMan software v7.1.0 (DNASTAR Inc., WI, U.S).
The presence of cognate EST and/or cDNA sequences provides proof of transcription for 40 (58%) of them (Additional File 1).
Resulting amplicons were separated on agarose gels, extracted and Sanger sequenced to proof their identity (Additional file 1: Figure S2).
Although we were able to identify a set of clones as more divergent even than the outgroup Nemorhaedus swinhoei, we were not able to cluster together all clones with unusual sequences, a proof that NuMts may be distributed throughout the phylogenetic tree, rendering phylogenetic detection not the most powerful diagnostic tool.
Sequences were proofed and edited using Geneious Pro.
Sequences were proofed and aligned by using CLUSTAL _X [ 36] as implemented in BioEdit [ 37].
Although we used traditional subcloning and Sanger sequencing for proof-of-principle, our method can easily be modified for high-throughput acquisition and analysis using bar-coded oligonucleotides and next-generation sequencing methods.
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