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The NCP-based substrate was cleaned by deionized water, ethanol, and isopropanol in sequence prior to use.
TatC, the largest and most conserved of three integral membrane components, provides the initial binding site of the signal sequence prior to pore assembly.
***While it is highly recommended to complete Physics sequence prior to enrolling at UC Berkeley, it is not required to declare the major and can be completed while enrolled at UC Berkeley.
This results in a predictive controller that performs a pre-determined heating sequence prior to the arrival of the substrate as part of the resulting feedforward/feedback strategy to eliminate the load disturbance.
A pre-tuning step was subsequently added to the production sequence prior to niobium sputtering to accommodate the frequency shift mainly due to mechanical tolerances during substrate production, substrate surface treatment, niobium sputtering and cooldown process.
The absence of relevant occurrences of Fagus sylvatica and Carpinus betulus in our sequence prior to 8800 cal BP, fail to give support to the glacial survival of these two taxa in the Apuseni Mountains, as hypothesized in the previous pollen and genetic studies.
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To minimize possible clashes between atoms of the crystallographic structure and the added missing peptide sequences, prior to homology modeling a short energy minimization step took place while keeping the crystallographic structures' heavy atoms spatially restrained.
Clones were verified by DNA sequencing prior to expression studies.
Fig. 2 Language Learners' Knowledge of Formulaic Sequences Prior to and Following Form-Focused Instruction.
The p132P, p120P and p90P constructs consist of the promoter and 5′ UTR sequences prior to the leader intron of atTOC132, atTOC120 and atTOC90, respectively.
All constructs were sequenced prior Leishmania transfection.
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