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These data show that Drosophila FCI-HDs have both shared (HD-common) and individual sequence preferences (HD-preferred) that differ markedly from each other.
Cleavage-site distance from the recessed end did not affect sequence preferences for M-MuLV reverse transcriptase.
The sequence preferences of retroviral RNase H likely reflect structural features in the substrate that favor cleavage and represent a novel specificity determinant to consider in drug design.
The helix-helix interactome space, with its associated sequence preferences and accompanying hydrogen-bonding patterns, should be useful for engineering, prediction, and design of protein structure.
The isolated RNase H domain of M-MuLV reverse transcriptase retained sequence preferences at positions + 1 and − 2 despite prolific cleavage in the absence of the polymerase domain.
Certainly, more shRNA-sequences from randomized libraries have to be analysed to determine general sequence preferences.
We discovered strong internal sequence preferences of peptide N-terminus trimming by ERAP1.
This impressive difference suggests that single positional side-chain preferences by ERAP1 can synergize to larger sequence preferences.
Overall, strong sequence preferences were clearly evident from this library screen even without a more detailed kinetic study.
The sequence preferences of CUGBP1 overlap with those of HuR and Pum1 proteins, both of which are also able to bind to elements with a core UGU trinucleotide.
We built a weight matrix describing the sequence preferences of Cbf1 from 16 Cbindingding sites characterized by Wieland et al.[18].[18]
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