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Tajima's D statistic [32] for each type of V3 sequence population was calculated using DnaSP 4.10 [4.10
The 406 sequence pair average was included as a control indicating the diversity within the 29 sequence population along the alignment.
Averaging the XOR scores for the functional sequences provides a threshold value for isolating putative functional sequences from the random genomic sequence population.
The variation between each patient sequence population to subtype A and subtype D populations were calculated using MEGA 4.0 (Figure 4A) and the Tamura 3-parameter molecular model using 1000 bootstrap replicates.
To determine whether HIV has exploited the lack of polymorphism of the proteasome and TAP, we predicted the number of epitope precursors in a HIV-1 clade B sequence population data set with samples from 1980 to 2005 (see Material & Methods for details on the HIV-1 Sequence Compendium data set [32] and the quality of the MHC-pathway model [21]).
The dN/dS ratio was calculated to determine the degree of selection in the sequence population.
Similar(43)
In addition, subtype A and D sequences formed distinct clades on the tree that were unrelated to patient sequence populations.
Although recombinants were often found in tissue sequence populations with high divergence, they were also found in sequence populations with a comparatively low sequence divergence (this is especially apparent in the two lymphoma cases).
For both sites, we did not detect any mixed sequence populations when analyzing the gene-specific amplicons (data not shown).
Overall, no significant genetic difference at the nucleic acid level between subtype A and D to patient sequence populations was found.
An entropy approach was used to compare two sequence populations and identify the significant (P<0.05) amino acid differences with randomization test [62].
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