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In this study, a rapid molecular marking method, gspCAPS (genome sequence pool CAPS), was established to develop soybean CAPS markers.
Training process is not needed in the coevolution mining methods, which align the query sequence against a large protein sequence pool to calculate the residue pair potential coevolution score.
The non-redundant sequence pool contained 1,864 sequences representing 743,527 bp of genomic sequence.
Hereafter we will refer to this combined set as the "accessory sequence pool".
The combined sequence pool from both community samples comprised 578 OTUs, 30.4% of which were shared.
Further, with increasing data set size, phred cutoffs limit erroneous sequences to a smaller sequence pool.
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Such sequencing 'holes' indicate regions that are absent from the respective strain or - in case of sequence pools - from a fraction of the pooled strains.
Plastid sequences in assembled and unassembled sequence pools were identified by BLAST searches [57].
In short, one can sequence pools of BACs in lieu of (or preferably, in addition to) random genomic DNA.
Sequences shorter than 150 base pairs (bp) were also removed from the final sequence pools.
(B) The similarity between two continuous sequence pools Seqm-1 and Seqm.
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