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The selection strategy used to obtain these KDEL-specific antibodies opens up ways to select antibodies to other conserved epitopes, such as the nuclear localization signal (NLS) or the peroxisomal targeting sequence, permitting the simultaneous analysis of specific groups of proteins.
Thus, the proteins become less and less bound to their original sequence permitting them to adopt other conformations and to fulfill additional or new functions.
Electrical stimulation (duration monophasic square wave pulses, 450 µs, frequency 30 Hz) was applied in coordinated sequence permitting cyclic patterns of muscle contractions resulting in leg cycling.
However, the pC-DEPN plasmid allows for insertion of fragments upstream of the GST coding sequence, permitting the expression of either native, untagged protein or C-terminal GST-fusion tags.
Populations that are not genetically distinct but experience only intermittent gene flow, such as those on islands, might be given a unique sequence permitting them to be specifically targeted by precision drives later on.
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Given a topology, the concept of failure sequence permits to generate three indicators, namely the overall robustness, its most sensitive components and the most threatening sequences.
The paragenetic sequence permits to schematically display the relationships between these diagenetic phases and the resulting porosity and permeability modifications (Moore 2001).
Use of a multicopy signature sequence permits detection of less than 1 genome equivalent per reaction.
The presence of variation in morphology, advertisement call, and DNA sequence permits questions about relationships among these suites of data.
As depicted in Figure 1, the TALEN-driven insertion of the reporter at the 3′ end of the Pmp22 coding sequence permits co-transcription of the reporter as part of the same RNA.
Deletion of this exon from the Mitf sequence permits the NLS to become the dominant factor in determining Mitf's subcellular localization and the protein become predominantly nuclear, as in the case of Mitf-dAB and Mitf-M.
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