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These rationale and availability of the rice genome sequence, online databases and in silico search tools enticed us to carry out a detailed analysis towards the identification and expression profiling of protein phosphatases in rice.
After a user submits a query sequence online, the " on-the-fly" pipeline will find the best matched gene family for the query, and insert it into the proper places within the existing phylogenetic tree and peptide alignment of the best matched gene family.
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The researchers say they will publish the crops' genome sequences online for anyone to download for free; they are also training hundreds of African plant breeders on how to use the data.
It converts output from the GenBank "Blast 2 Sequences" online alignment software (www.ncbi.nlm.nih.gov) to a manageable database of identified sequence variants.
The methylation level of the FOXP3 promoter region during transient expression was also determined in one donor using bisulphite sequencing (online supplemental material Figure S3A).
GO analysis was completed by submitting gene sequences online (see Materials and Methods).
GCK mutations were confirmed by direct sequencing (online appendix Table 1 [available at http://dx.doi.org/10.2337/dc07-2017]).
Search engines usually need to digest protein sequences online as well as filter peptides according to the mass error, which may add unnecessary overhead.
Furthermore, six PSYs were retained in B. napus; however, we selected only three B. napus PSY genes that had complete sequences online.
And the following is a sequence of online banner ads: "Are you a jug addict?
Including my flubs, I probably put in 12 to 15 hours of filming for a 45-second sequence, viewable online at www.nytimes.com/personaltech/.
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