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In order to harness the comprehensive sequence manipulation features of BioJava libraries, we extended the BioJava 3.0 Core DNASequence object for the JEnsembl DNASequence object, providing an Ensembl SequenceReader that can lazy-load sequence on demand from the Ensembl datasource.
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Various proxy implementations are provided which can store sequences in memory, fetch sequences on demand from a web service such as UniProt or read sequences from a FASTA file as needed.
To obtain an optimal expression of IncA in human cells full-length DNA sequence of IncA of Cp. psittaci was optimized for human codon usage (sequence available on demand), in vitro synthesized (GenScript) and cloned as an EcoRI-NotI-fragment into pcDNA3.1/myc-His A (Invitrogen).
Thus, a personalised therapy concept that integrates individualised tumour genome sequencing and on-demand RNA vaccine manufacture to create customised drug products is both an exciting new therapeutic vision and is within our grasp (Kreiter et al, 2011b).
This question can only truly be answered if we are able to sequence individual genomes on demand and then, only if it is cost effective.
All primer sequences are provided on demand and primers were purchased from Eurofins MWG Operon (Ebersberg, Germany).
This system may enable researchers and physicians to remotely regulate the timing, dose, and sequence of drug delivery on-demand, with a wide range of clinical applications ranging from tissue engineering to cancer treatment.
Primers and probe sequences from the Assay-on-demand (Applied Biosystems) are not revealed by the manufacturer.
A pET-N-Sac was derived from pET-N by introducing a SacI restriction site at the end of the N-coding sequence by using the Pfu DNA polymerase with the QuickChange Site-Directed Mutagenesis Kit (Stratagene) according to the manufacturer's instructions (sequence of primers available on demand).
mRNA silencing was quantified by real-time PCR with an ABI PRISM 7900HT Sequence Detection System and Assays-on-Demand™ gene expression products as described previously [13].
: SNP s) are located under a primer or probe sequence Since we used assays-on-demand, we wanted to verify performance of the primer and probe sets.
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