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As for [I], since B a (posterior ancestor) is sampled from the reference sequence (offspring) B1 and the integrand is the offspring (B2, B3) probability derived from the representative ancestor B a which is already linked to offspring B1 through posterior sampling.
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Pairs sequenced: Offspring estimated heterozygosity of sequenced pairs 19.
Sequences from offspring were sequenced and genotyped and these data used for genetic mapping.
Assessing whether the sequence of offspring is correctly imputed can only be done if a sample of such offspring is actually sequenced, and such data are presently not available in sufficient quantities.
We investigated the relation between laying date, the position of the egg in the laying sequence, and offspring sex ratio in two closely related avian species, the Wood pigeon and the Rock pigeon.
The 20 genomes of five complete tetrads were sequenced at an average coverage of 54x, whereas eight other complete tetrads were sequenced at lower average coverage of 14x, amounting to a total of 52 sequenced tetrad offspring (Supplementary file 1A).
Lastly, if sequencing additional offspring is an option, statistical power can be improved.
DOI: http://dx.doi.org/10.7554/eLife.01426.009 10.7554/eLiFigure2 figureigure 2—figure supplement 4. The number of putative type-1 and type-2 NCO GCs in all 20 deeply sequenced tetrad offspring using different marker sets.
With the final set of 137,339 markers, we genotyped all 20 deeply sequenced tetrad offspring for the presence of putative NCO GCs at markers that were not found to be involved in CO-associated gene conversion (Supplementary file 2B, 'Materials and methods').
This tree describes how the offspring sequences descend from the source sequence.
Because homologous recombination was seen in all progeny--and not in just a fraction of plants as would be expected if classic Mendelian genetics was operating the team says that this unusual transmission is not simply a case of a parent passing a modified DNA sequence to its offspring.
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CEO of Professional Science Editing for Scientists @ prosciediting.com