At the extremes this is already occurring in that for the most precious samples there is tendency to store very raw data (e.g., the Solexa images for ancient DNA samples) and there is not the expectation by authors or manuscript reviewers to, say, submit the sequence of "sequence verification" on cloning vectors.
The earlier paper of this series (2004d, Powder Technology 143 144, 65 83) illustrated a sequence of development and verification work on a breakage model for FBMG, based on the population balance modelling work on tracer experimental studies.
Mutant plasmids were ratified by sequencing, and then the mutagenised insert was sub-cloned back into the wide host-range plasmid pMP220 to form pBIO1918, which was sequenced for verification of the mutation.
This technology validates PCR-based assays by qualitatively verifying that a positive PCR result is not the effect of nonspecific amplification, as shown here by sequence verification of PCR amplicon generated from virulence plasmids pXO1 and pXO2.
Presence of the virulence plasmids was determined by PCR and sequence verification of regions on plasmids pXO1 and pXO2 (Table 1).
Through an integrated approach, in situ inspection, testing, numerical and analytical modelling are associated under the scope of reproducing the existing structural damage, the sequence of inelastic behavior and verification of the necessity of retrofitting measures.
Sequence verification of PCR-generated fragments was performed by sequencing.
Whenever necessary, sequence verification of plasmid constructions was carried out on an automated 3730 DNA Analyzer (Applied Biosystems).
Primer specificity was confirmed by agarose gel electrophoresis, melting curve analysis, standard curve analysis, and sequence verification of cloned PCR amplicons.
Primer specificity was confirmed by agarose gel electrophoresis, melting curve analysis and standard curve analysis, and by sequence verification of cloned PCR amplicons.
