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Nucleotide differences at the first position in RSIV CH-1 prevented the binding of the sense primer derived from the sequence of the reference strain (RSIV Ehime-1) and appeared to cause a negative result in PCR amplification of the targeted Pst I restriction fragment.
where Δ0i (k) is the deviation sequence of the reference sequence ( Z_{0}^ (k) ) and the comparability sequence ( Z_{i}^ (k) ), ζ is distinguishing or identification coefficient.
Now, Δ0i (k) is the deviation sequence of the reference sequence ( Z_{0}^ (k) ) and the comparability sequence ( Z_{i}^ (k) ) is shown in Table 8.
Roche aligned sequence reads for the eight strains against the sequence of the reference strain E. coli K12 MG1655 (NC_000913).
During the matching pipeline in the SOLiD™ System Analysis Pipeline Tool, reads are automatically mapped to both the forward and reverse-complement sequence of the reference.
For reconstruction of C. difficile pathways, the complete genome sequence of the reference strain C. difficile 630 was downloaded from Genbank (accession number AM180355).
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To account for possible frame shifts caused by sequencing errors in ESTs we use genewise [ 25] to generate a codon-alignment for the EST and the protein sequence of the reference-taxon.
In the pileup files each line represents a sequence position of the reference sequences, consisting of the sequence name, position, reference base, number of reads covering the site, read bases and base qualities.
CDS sequences of the reference strains were obtained from NCBI according to the RefSeq ID.
Our data and parameters were: CDS sequences of the reference genome, MG1655 (NC_000913); genomic sequence of contigs assembled de novo by Roche using Newbler; blastn with default parameters; e-value cutoff 0.0001; DAGChainer options −g 5 −D 20 −A 5.
We also compared their predicted amino acid sequences with the respective sequences of the reference strain.
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