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Therefore, fitness increase was not directly correlated with the number of mutations in the consensus sequence, and in some clones, this fitness increase could not be linked to any mutation in the consensus sequence of the population.
In order to quantify collective properties of the molecular ensemble, we calculate the consensus sequence of the population.
The consensus sequence of the population of T7LME_2000 contained 17 mutations in addition to those found in T7 LME_200, indicating that evolution continued but that fitness effects per mutation were small by usual standards of T7 adaptation (Table 3).
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A total of 20.4 M Illumina reads were obtained from sequencing of the population.
Full resolution of haplotype information ultimately requires sequencing of the population.
The bi-filtering method was also suitable for analyzing the genotyping data by re-sequencing of the population and parents.
In this case, we would get the sequences of the population of endoparasites (presenting thus some distinct but related haplotypes) and the one for the bruchid?
Sequences of the population at ~160 gens (40 hr) identified two changes greater than 90% frequency and two others near 30% (Table 2).
Protease substitutions I13V, E35D, M36I, R57K, H69K, and L89M, which serve as drug-resistance support mutations in subtype B, were present in the majority of subtype-A1 sequences of the population.
Protease substitutions I13V, E35D, M36I, R57K, H69K, and L89M, which are drug-resistance support mutations in subtype B, were present in the majority of subtype-A1 sequences of the population studied, suggesting that these sequence alterations may occur as natural polymorphisms, and may serve as genetic signatures.
The analysis of the consensus sequence of this population revealed that none of the polymorphic mutations previously identified was fixed at transfer number 90.
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