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We also included in our analysis the sequence of region B from Z. bailii [12].
The nucleotide sequence of region B from strain 59A was deposited in EMBL-GenBank under accession number HQ615872.
Furthermore, two copies of an 11 bp ARS consensus sequence (ACS, 5'-WTTTAYRTTTW-3') were identified in the sequence of region B, at position 8524 to 8535, referring to the circular form, (ACS1, ATTTATATTTT) and 13763 to 13774 (ACS2, TTTTATATTTT).
This provided the sequence of region C and also repetitive sequences inserted at the breakpoint junction but not region A. The rest of the AC region was isolated by PCR using two primers, NR located at the end of the λz3-96 clone and AF anchored in gene CG2046 from region A (Figure 1).
sequence of 3' region of preproFRMFa cDNA.
The sequence of region C was found to contain two genes with nonsynonymous changes: APJ1 (YNL077W) and NIS1 (YNIS18W).
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The control objective is to make a group of mobile agents cover and sense a sequence of regions of interest.
In addition, homology evaluation using BLAST for isoforms of human MUC1 showed that the 19 aa sequence of Region-5 was identical in 45 isoforms, whereas the 19 aa sequence in Region-3 was identical in 35 isoforms [ 14].
In order to verify the nucleotide sequence of regions harboring putative SNPs we performed local re-sequencing of the genomic DNA.
Furthermore, generating the sequence of regions within captured gaps (e.g., during human-grade sequence finishing) is most readily achieved by sequencing the spanning plasmid subclones; in contrast, generating the sequence of regions within uncaptured gaps requires sequencing alternative templates (e.g., PCR products or purified BAC DNA), which is more costly and labor-intensive.
The sequences of region B and of the homologous region in Z. bailii are almost identical (99.7%), suggesting that this introgression event is recent.
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