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Because pmpD is highly conserved among all reference strains [12], constructing rPmpD using the pmpD sequence of reference strain E/Bour should not have contributed to the observed differences in immunoreactivity.
By filtering the alignments with respect to the active residues marked in the sequence of reference protease and the localization prediction, we obtained 47 class A, 39 class B and 32 class C proteins (vide supra) and their corresponding genes.
These 2 sequences are different from the gnd sequence of reference strain STEC O91 H21.
* Numbering according to nucleotide sequence of reference SRLV strain MVV KV1514 [GenBank accession number: M10608].
*p > 0.05 is consistent with the Hardy-Weinberg equilibrium; ** Sequence of reference NT_010194.16.
* Numbering according to nucleotide sequence of reference SRLV strain CAEV Co [GenBank accession number: M33677].
Recently, the increase of complete or partial genomic sequence of reference plant species from different families and the development of the BAC clones sequencing in experimental species gave the opportunity to perform direct comparisons at the genomic sequence level.
In order to establish the extent of genetic diversity in natural populations of this microbe, the genomic sequence of reference strain DSM 13855 was compared to metagenomic fragments recovered from climax saltern crystallizers and obtained with 454 sequencing technology.
The minimal length of discrimination between sequences of reference strains is for the V1 region described in Fig. 2. Positions refers to E. coli numbering[ 11].
Sequences of reference strains obtained from DNA databases are indicated in bold and accession numbers are given in parenthesis.
A phylogenetic tree was created with the gene sequence of strain CCG1 and with their similar sequences of reference strain which are obtained from NCBI database.
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