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We report the ∼1.79 Gb draft whole genome sequence of reference genotype Tift 23D2B1-P1-P5, which contanns an estimated 38,579 genes.
Based on the whole genome sequence of reference genome Nipponbare, TIGR prediction method (http://rice.plantbiology.msu.edu/), available information from the 3000 Rice Genome Project (3 K RGP), and OryzaSNP (http://snp-seek.irri.org/) database, 27 putative InDel markers were designed to narrow down the BPH31 locus and were surveyed for polymorphism between Jaya and CR2711 76.
Because pmpD is highly conserved among all reference strains [12], constructing rPmpD using the pmpD sequence of reference strain E/Bour should not have contributed to the observed differences in immunoreactivity.
By filtering the alignments with respect to the active residues marked in the sequence of reference protease and the localization prediction, we obtained 47 class A, 39 class B and 32 class C proteins (vide supra) and their corresponding genes.
Using the primers CAP-F (5'-CGTGTATGCAAGTACATTAC-3') and CAP-R (5'-CTGATTAGTCATTAGTCCATC-3'), we amplified a fragment of 598 bp (without primers) that corresponds to the positions 15,653 to 16,250 on the complete goat mitochondrial sequence of reference ([42]; accession number AF533441).
* Numbering according to nucleotide sequence of reference SRLV strain MVV KV1514 [GenBank accession number: M10608].
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The minimal length of discrimination between sequences of reference strains is for the V1 region described in Fig. 2. Positions refers to E. coli numbering[ 11].
Sequences of reference strains obtained from DNA databases are indicated in bold and accession numbers are given in parenthesis.
A phylogenetic tree was created with the gene sequence of strain CCG1 and with their similar sequences of reference strain which are obtained from NCBI database.
The final sequences were compared to the nucleotide sequences of reference organisms available in the GenBank database using Blastn (Altschul et al. [1990]).
Whatever the reason, the sequencing of reference genomes provides us with a new way to see life and, through comparisons between and amongst species, offers a deeper and richer understanding of the world we live in.
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