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With regard to the determination of the sequence of recognition of the anti-33-mer moAbs (G12 and A1), a positive signal was detected only in the bacterial extracts containing the fusion peptides Pro63-Tyr69 (PQPQLPY) and Gln64-Pro70 (QPQLPYP) for the G12 moAb and in the bacterial extracts containing the fusion peptide Gln66-Pro72 (QLPYPQP) for the A1 moAb (Figure 3B).
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Peptide-induced AgNPs assembly was achieved on the electrode surface that was modified with the same sequence of peptide (recognition element) used in the AgNPs aggregation.
However, the exact amino acid sequence of the recognition sites by these two sheddases is unknown.
The fusion construct of AnkR_AS and AnkB_repeats was made by standard two-step PCR with a coding sequence of thrombin recognition residues 'GSLVPRGS' as the linker.
In eIF4E family members capable of binding eIF4G, the consensus sequence of the recognition motif is S/TVxxFW ending at W73 [ 31, 32, 56- 58].
Interestingly, there are instances in beetles of frame shift mutations causing the mTERF site to become part of the coding region despite no changes to the sequence of the recognition site [ 70].
One area that was not directly examined in this study but would likely improve data quality is the use of restriction enzymes that are biased away from repetitive regions by the sequence of their recognition motif.
Fourth, knowledge of signature peptide sequences for recognition of specific modified nucleosides could provide clues as to how best to narrow bioinformatic searches for modification enzyme functions.
This could possibly be due to the different lengths of the duplexes and flanking sequences of the recognition site, which might affect the methylation activity.
Filopodium-mediated osteoclast fusion illustrates the sequence of cell-cell recognition, an intermediate stage, fusion of the cell bodies, and the reshaping of the fused cell (Fig. 3A).
Previous analysis of the Glossina fat body transcriptome uncovered the sequence of a pathogen recognition protein implicated in the initiation of innate defense mechanisms [ 29].
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CEO of Professional Science Editing for Scientists @ prosciediting.com