Since the 1990s small electric arms have become important in molecular biology laboratories, precisely handling test-tube arrays and pipetting intricate sequences of reagents.
The literature gives little guidance on the factors (e.g. choice of reactor, sequence of adding reagents, imperfect mixing) determining the product distribution of parallel reactors.
By considering two irreversible, second-order reactions between three substances, it is shown here how the sequence of adding reagents to a semi-batch reactor will influence the yield when the mixing is perfect as well as when segregation is complete.
If the sequence of the reagent is not reported in a paper, curators may need to contact authors to request the necessary information.
The primer sequences for making double-stranded long RNAi reagents were collected and amplicon sequences of RNAi reagents were assembled by virtual PCR based on FlyBase release 5.51 using UP-TORR (Hu et al. 2013).
The primer sequences from FlyPrimerBank were blasted against the sequences of RNAi reagents and the overlap of each primer sequence with RNAi reagents was analyzed using a program developed in-house in Java.
Since the DNA extraction process based on solid phase extraction involves multiple sequential steps (i.e., loading, washing, and elution), an automated electromagnetically controlled valve-array was designed and used to control the flow sequence of the required reagents and washing buffer.
Several recent studies demonstrated that the degradation of intended transcripts by siRNA (so-called "on-targets") and unintended effects arising from inadvertent targets (so-called "off-targets") depend on the sequence of the RNAi reagent and have to be computationally analyzed [ 4, 5].
Indeed, all phylogenomic methods require significant financial resources, but when factoring in the costs of reagents, sequencing, and labor versus the number of nucleotides sequenced, target enrichment type methods are far less expensive than traditional PCR-based methods [ 9].
Despite the drop in the cost of sequencing reagents, the complete study of a genome, from sampling to finishing the assembly is still costly and difficult.
