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Based on the calculated sequence coverage and comparison with other Pseudomonas genomes (see below), we believe that the vast majority of genes are present in the current draft, though the draft genome sequence of race 4 is not as complete as that of B076.
Primers for G10H and STR genes cloning were designed according to the entire assembled sequence of RACE PCR.
A detailed analysis of class I and II TEs identified in the near complete genome sequence of race O was performed.
A PCR primer pair was designed to amplify a full-length putative ORF region of the core clock and photoreceptor genes using the Oligo software program based on the sequence of RACE products.
BGT acknowledges, with gratitude, the US Department of Energy Joint Genome Institute (JGI) for their fungal genome program, in particular, for their support in generating the sequence of race O, strain C5, of Cochliobolus heterostrophus http://genome.jgi-psf.org/CocheC5_1/CocheC5_1.home .jgi-psf.org/CocheC5_1/CocheC5_1.home
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Vettel suffered a spark plug problem that proved terminal and it followed a sequence of races in which the championship has swung firmly in Hamilton's favour.
The Mercedes driver secured such a strong lead after a sequence of races where he and his team have repeatedly proved to have the edge on Vettel and Ferrari.
Sequencing of RACE and RT-PCR products confirmed the organization of these two genes.
RACE products were amplified (sequences of RACE primers are available upon request) using 2 μg of a pool of total RNA from different CS tissues (see Plant material).
The 3′ and 5′ end nucleotide sequences of RACE-PCR products were used for the priming sites for the full-length cDNA in RT-PCR.
The sequence of this 3' RACE clone was identical in the area of overlap with the single class I fragment obtained from 5' RACE.
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