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Another problem is that the complete genomic sequence of pigs is not available, and many miRNAs that do not align to the pig genome cannot be identified by miRDeep.
Next, the remaining pre-miRNAs were blasted with the genome sequence of pigs and the sequence fragments (coverage >85%, identities >85% with pre-miRNAs) were retrieved from genome.
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In this study, we determined the cDNA sequence of pig testicular 17β-HSD Type3.
In this paper we describe the first exome sequencing of pigs.
A high sequence similarity (>99%) between NV sequences of pigs and humans was noted after aligning the whole genome sequences.
We are unable, at this time, to exploit complete genome sequences of pigs or other closely related taxa to discuss the impact of domestication on aromatase, steroid receptors, amphiregulins, or other proteins that appear to be associated with uterine capacity and large litter sizes in the domesticated pig [ 75].
As the sequencing of pig Y chromosome fosmids progressed [ 1], it became clear that some sequences were present at a high copy number.
Also shown are the numbers that had unique Gene IDs in the NCBI HomoloGene database (a database of orthologs among species) and corresponded to the sequences of pig cDNA clones.
The numbers of unique Gene IDs corresponding to sequences of pig cDNA clones that were not mapped at any locations on the draft pig genome sequence (Sscrofa10.2) are also shown.
The cDNA libraries that we used included seven libraries constructed by using animals that were cloned from a sow used for draft sequencing of pig genome (Duroc 2 14) [ 5- 7].
A major hurdle in the validation of the Pig-a assay has been confirmation of the mutational basis for the observed phenotypes (i.e., GPI-anchor deficiency); however, sequencing of Pig-a mutant blood cells is not possible since RBCs lack nuclear DNA.
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Justyna Jupowicz-Kozak
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