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The aim of the strategy was to insert a mutant tub2 gene copy (either SA or SE) into its normal locus (see Materials and Methods, and Figure 1A for partial amino-acid sequence of mutants).
Even the sequence of mutants with recessive phenotypes compared with the wild type progenitor may not answer the question of functionality.
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Despite increasing interest, no consensus target sequence of mutant p53 has been defined so far.
Table 8 gives the location and sequence of mutant substitutions and Figure 11 depicts mutant locations with respect to the secondary structure of the introns we studied.
Each mutagenic oligonucleotide contained 15 base pairs of complimentarity to lyz cDNA on either side of the target codon; the sequence of mutant codons was selected based on preferred S. cerevisiae codon usage.
For the construct ΔSP-GLP-1R-GFP, forward primer with a start codon was designed to clone the coding sequence of mutant GLP-1R without the first 23 amino acids.
The primer sequences of mutants are shown below.
One approach for determining the mode of action of a small molecule involves the selection and full genome sequencing of mutants that acquire compound resistance.
Interestingly, Cheng et al. (2000) reported that sequencing of mutants from chromium-treated mice revealed a large increase in GC to TA transversions.
Sequencing of mutants from the monkey using reverse transcriptase polymerase chain reaction methods has led us to conclude that there is similarity of the spectra observed between the spontaneous mutations detected in the two species.
Obtained sequence coverage of mutants.
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