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The sequence of markers in the TN DH linkage map was used as a query sequence.
A (maximal) syntenic block of size k in genome X with respect to genome Y is (maximal) sequence of markers (xi.... xi+k) not containing any breakpoint.
All forward primer sequence of markers were labeled with four fluorescent dyes of different colors [FAM (blue), HEX (green), TAMRA (yellow), and ROX (red)].
DArT markers can easily be sequenced and to obtain stronger support for the microsynteny of the QTLs (or genes) for waterlogging tolerance among grass species, further research should involve direct comparison of DNA sequence of markers (those linked to QTLs) to that of the genome sequence of rice [ 54, 55] and other species.
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Adult hippocampal neurogenesis in mice and rats shows a sequence of marker expressions that has been described in increasing detail [17], [40], [44], [48], [49].
The sequence of marker 12H3 is predicted to encode an ATP dependent DNA helicase/uvr/REP helicase, an enzyme that makes single stranded DNA from double stranded DNA.
We further find out that HAP4 co-expresses well with these genes if the sequence of marker block 41 is inherited from RM strain.
The primers and sequences of markers corresponding to these disease resistance QTLs were obtained from the Cotton Marker Database (http://www.cottonmarker.org).org
12284_2014_2_MOESM1_ESM.xlsx Additional file 1: Primer sequences of markers developed for the genetic analysis.
The set also included sequences of markers that are directly used in mapping.
DNA sequences of markers have been submitted to Genbank under accession codes FH893713 – FH893713.
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