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The sequence of integrated TUB2 (WT) or tub2 (SA or SE) genes was checked after PCR amplification of a 1.8 kb genomic DNA fragment containing the TUB2 gene (primers used for PCR amplification were as in [23]).
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The selection of possible distillation columns sequences using rising values of the integrability criterion gave a very good approximation towards the best sequence of heat integrated distillation columns when compared to their total annual cost.
Clones of temperate, caespitose grasses were organized as assemblages of autonomous ramet hierarchies, rather than as a sequence of completely integrated ramets.
This effect did not interact with group (p=0.75), suggesting that although the effect was less pronounced for left-hand-trained participants, the distribution of integrated and independent sequence encoding was similar for left and right hand sequence production.
Results show interesting trends in the optimal designs that integrated the trains in energy consumption and that the controllability properties of integrated distillation sequences may change significantly depending on the selected operation point control properties.
In addition, we detected two copies of complete sequences of PERV integrated into the genome (Table S17 in Additional file 1). Figure 4B shows the evidence of the integration of a copy of PERV in the middle of the assembled scaffold, and the integrating points were well supported by paired-end reads.
For CB13, we were able to characterize sequences for plasmids QpH1 and QpDV, which can be caused by several situations: this isolate may have 1) 2 different plasmids, 2) a QpH1 plasmid and sequences of QpDV integrated in the chromosome, or 3) a new plasmid that arose from combination of QpH1 and QpDV.
In B. malayi, a large amount of sequence integrated into the nematode nuclear genome from its obligate mutualist Wolbachia endosymbiont relatively recently such that it has not diverged significantly [ 5, 6], which further complicates assembly, resulting in numerous collapsed repeats and corresponding gaps in the assembly [ 5].
A direct comparison of the inactivation status of sequences integrated into the same genomic location would permit a direct analysis of the impact of cis-acting sequences on XCI and potentially identify sequences containing escape elements, waystations, and/or boundary elements.
By the analysis of HPV-18 transcripts, we observed the diversity of HPV-18 splicing of sequences integrated in the host genome.
This comparison indicated they were of high quality, demonstrating the added value of integrating sequence- and expression information for gene function prediction [ 34].
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