Sentence examples for sequence of inserts from inspiring English sources
Appropriate frame and sequence of inserts was confirmed using an ABI Prism 3730xl DNA Sequencer (Genewiz, South Plainfield, NJ, USA).
The DNA sequence of inserts was analyzed by Eurofins MWG Operon (Berlin) with the primer-walking technique.
The sequence of inserts was determined by sequencing using specific primers and compared with the data base of the NCBI using the BLAST program.
Further, to investigate the accuracy of the PCR and the ligation reactions, we pick up 4 of 9 correct plasmids as examples, and determined the sequence of inserts within the arrays containing 2 kb segments.
All generated recombinant plasmids were sequenced (GATC, Konstanz, Germany) to ensure correct orientations and sequences of inserts.
Colony PCR and DNA sequencing of inserts from recovered clones after ligation and transformation demonstrated an even distribution of construct sizes (Fig. 1B).
The nucleotide sequences of inserts were verified by automatic sequencing.
The sequences of inserts of pGL3-T and pGL3-G were confirmed by direct sequencing.
Furthermore, the secretome selection protocol was combined with the next-generation sequencing of inserts.
These 6055 cDNA clones were served as a basis for complete sequencing of inserts, 5006 of which were successfully sequenced.
The most useful transcript sequences are derived from high quality full-length sequencing of inserts from cDNA clones (FLIcs) that contain the complete protein coding sequence (cCDS).
