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Such mutations could include not only changes in the sequence of existing microRNAs, but also the emergence of new hairpins within the same transcript, especially when their seeds are identical to those of ancestral members.
We have presented a stochastic evolution model for protein networks, which is based on fast link dynamics due to mutations of the coding sequence of existing proteins and a slow growth dynamics through gene duplications.
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The primer sequences of existing clonality assays were aligned to the reference sequences of all rearranged genes and genes were scored as to the likelihood of being recognized by a primer.
Furthermore we compared the primer sequences of existing RT-PCR assays to the genome of this variant strain.
Approximately two-thirds of the contigs had significant matches against sequences of existing proteins and 9% were annotated using Gene Ontology terms.
36 Many questions have arisen as to the appropriate sequencing of existing and novel therapies with Ra and if/how to combine Ra with these systemic therapies.
Bacteria can evolve by changes to the sequences of existing genes and regulatory regions and by changes to their gene content.
These lineage-specific apiAP2 genes in the present-day species could have arisen from de novo gene birth or modification of the full-length sequences of existing genes beyond recognition.
This positional information could be useful in detecting CNEs over large evolutionary distances, under the assumption that evolution sometimes proceeds by modifying the sequences of existing cis-regulatory CNEs without significantly changing their relative position [ 31].
Sequencing of normalized libraries, or more intensive sequencing of existing libraries, perhaps with newer generation of pyrosequencing methodology, may increase the number of salivary secreted peptides, but possibly to no more than 10% of the number found with random sequencing of ~2,000 clones.
Additional file 1: Statistics and sequence labeling of existing datasets.
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