Newer sequencing technologies, offering rapid high throughput sequencing, reduction of sequencing cost, and complementary software have changed the situation dramatically, making these methods more readily considered as standard molecular tools for biology and medicine [ 1- 9].
With the rapid reduction of sequencing cost, sequence data will soon be available for the use in breeding programs.
With the reduction of sequencing cost, sequence based whole genome expression profiling could be used to characterize increased SBS frequency in patient's tumor and aid diagnostic.
With the progress of the new generations of sequencing techniques, the cost of sequencing a bacterial genome will soon drop below that of an average phenotyping experiment and the number of sequenced prokaryotic genomes keeps growing rapidly.
A bigger impact of the reduced sequencing cost of second-generation sequencing methods is the ability to generate much more transcript sequence than ever before.
One such tool is the MACC (marginal abatement cost curve), used to capture the least-cost sequence of abatement options.
For our illustration, we calculated 95% confidence intervals for a sequence of 10 uniquely spaced cost values.
Additionally, improvements in exonic enrichment protocols will usher in an era of cost effective sequencing of all the amino acid coding bases of genomes.
In terms of cost, the sequencing method costs $50 per exon, so $150 for three exons per specimen.
With the continuous decrease of sequencing cost, RNA-seq become the most prevalent method for identification of circRNAs.
