With the error rate q i,j,d, the upper probability (P) that the number of reads (R) with a certain sequence alteration of category j in position i is equal or greater than the number of observed reads r out of N reads with a sequenced direction d for an unknown sample was defined as: (2) where, λi,j,d = N i,d × q i,j,d.
The alternative hypothesis is that the sample means change monotonically along the ordered sequence of the categories.
However, to be in line with a recently published version [ 31], the sequence of response categories were changed, as shown in Additional file 1.
The first sequence of each species category in the MSA is the sequence from that category with highest alignment score.
We generated sets of 1000 shuffled sequences for each sequence category in every one of the genomes analyzed.
This highlights the importance of category sequence information for the task.
The reinforcer devaluation task requires the monkey to be sensitive to the hedonic value of food in the mouth (rather than simply obtaining a reward or not), which is experienced only after the object has been chosen and the reward taken, whereas the strategy implementation task clearly requires the monkey to be sensitive to the sequence of choices among categories of visual objects.
In contrast, the less frequent non-canonical PAS (Table S2) were distributed equally in the 3'UTR sequences of all categories (Fig. 8A).
However further studies are needed using more than one video sequence per category of pain severity to confirm this preliminary finding.
A progressive sequence of capabilities for each category starts with its earliest feasible missions and leads toward its full expression.
