A bead movement was included in the data set only when the complete sequence of bead binding to the microtubule, processive movement, and release was observed, and only movements longer than a minimum length needed for reliable detection (225 and 500 nm for heterodimer and wild-type, respectively) were used for subsequent quantitative analysis.
Previously drawn beads were displayed throughout the trial, to reduce working memory demands, and the sequence of drawn beads was fixed across all participants.
We adopt a simplified molecular model for polymethylene-like chains; the chain is made of CH2-like beads connected by harmonic springs, and the lowest energy conformation is a linear stretched sequence of the beads with slight bending stiffness being imposed.
All atomic details were suppressed and the chain was represented as a sequence of identical beads.
As lunar mountains protrude onto the hairline-thin ring of the sun, it will be seen not as an unbroken ring but an irregular, changing, sparkling sequence of arcs, beads and diamonds very briefly encircling the moon: a "diamond necklace" effect!
For validation, the two examiners were tested using a random sequence of hidden beads.
Van Lehn et al. increased the speed of the simulations by treating the nascent protein chain as a sequence of coarse-grained beads, with each bead representing several amino acids.
Further, this report illustrates the possibility of fishing out specific sequences carried on beads using FACS, a feature that could be used for colour-coded MID (Multiplex Identifier, Roche/454 tag -specific FACS enrichmentag -specifico load and sequence optimal numbers oFACSads carrying enrichmentag.
All published studies that use the MARTINI coarse-grained forcefield to study how Ras proteins interact with lipid bilayers assume that farnesyl, a complex unsaturated lipid, can be represented as a linear sequence of 3 hydrophobic beads.
This single stranded library is then tested for optimal sequencing parameter through generation of sequencing beads by emulsion PCR with dilutions of the single stranded library.
