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An IR is a maximal sequence of alignment columns having at least one gap character each and flanked by gapless columns.
The sequence of alignment of R. pseudochrysanthum s.l. had 585 bp, including 41 polymorphic sites, of which 15 were parsimony-informative.
We represent one alignment A between two proteins as a sequence of alignment states, where L is the alignment length and is the alignment state at position i.
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In order to ensure that sequences of alignment are representative and diverse, we collected the homologue sequences by using seventeen different sequences as initial query sequences.
The primers used for the qPCR were designed based on the consensus sequence of each alignment.
Sequence logos of alignments were generated with WebLogo [ 72].
The number of sequences of an alignment does not influence prediction performance significantly.
It contains all sequences of the alignment and is ignored during the prediction of SDPs.
Consensus sequences of the alignments were then aligned with the corresponding transposon from M. murinus.
Nucleotide sequences of these alignments were then aligned codon by codon, using the PAL2NAL script [ 49].
We provide FASTA files of concatenated sequence alignments of the above filtered loci datasets.
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