Exact(5)
The genome sequence of a reference strain (SN15) was shotgun-sequenced using Sanger technology and assembled into 107 nuclear scaffolds and the complete mitochondrial genome (Hane et al. 2007).
Multiple species of bacteria and hundreds of strains thereof are being sequenced every year, thanks to cutting edge approaches such as re-sequencing wherein genome sequence of a reference organism is used as a scaffold to direct analysis of several different strains [1].
In these comparisons, the sequences of one or several newly sequenced genomes are compared to the sequence of a reference genome.
The sequence of a viral protein can be highly divergent from the sequence of a reference protein, and this could be responsible for the loss or gain of interactions.
However, if enough substitutions have occurred such that multiple subsequent substitutions occur at the same position, the distribution of ΔS computed based on the sequence of a reference species or inferred ancestral sequence will no longer be exact.
Similar(55)
Instead of assigning taxonomy based on a range of matches to sequences from a set of reference genomes, SPANNER considers the similarity of the overall LCA Profile of a sequence to a reference database of LCA Profiles similarly constructed from the database of reference genomes.
In contrast to other published methods, readDepth does not require the sequencing of a reference sample, and uses a robust statistical model that accounts for overdispersed data.
Here, we evaluated how assemblies made with such improvements compare with assemblies produced with Sanger data, and how a mix of Roche/454 and Solexa/Illumina technologies performed in whole-genome sequencing of a reference bacterial genome.
To align a group of sequences to a reference alignment, we used the "--add" option of MAFFT [ 94].
Mapping sequences to a reference sequence of G. spinigerum, (GenBank accession no. AB181155) comprising partial 18S, entire ITS-1, 5.8S, ITS-2, and partial 28S regions, demonstrated our sequences (see Figure 2 for accession nos).
The de novo assembly of 1 billion DNA sequences to a reference genome of 393 Mb length provides an unprecedented insight into the I. ricinus genome.
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