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Mutations are changes in the DNA sequence of a cell's genome.
In the mock photolysis experiment (Fig. 2A), the IRM imaging sequence of a cell crawling across a FBN path did not differ from that of a control cell undergoing random migration.
The cDNA of the Os4bglu12 gene, which encodes a protein identical at 40 of 44 amino acid residues with the N-terminal sequence of a cell wall-bound enzyme previously purified from germinating rice, was isolated by RT-PCR from rice seedlings.
The genome sequence of a cell line is a comprehensive basis for many genetic characteristics of the cell line, it is closely relevant to other omics approaches such as transcriptomics and proteomics on the cell line, and also facilitates targeted genome editing of the cell line.
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Matsuoka et al. [ 35] report on the EST sequencing of a cell suspension library of tobacco, which was the origin of a large portion of the tobacco gene index.
The transcribed sequences of a cell, the transcriptome, represent the trans-acting fraction of the genetic information, yet eukaryotic cDNA libraries are typically made from only the poly-adenylated fraction.
Using exome sequencing of a cell line derived from the spontaneous mouse melanoma B16, Castle et al. uncovered tens of neo-epitopes generated by mis-sense mutations and characterized them with respect to their immunogenicity; they observed that a significant proportion of the predicted neo-epitopes was actually immunogenic in vivo.
We then used our results to deduce a temporal sequence of cell to cell interactions and representative signaling events in the retina following optic nerve injury.
Upper right panel shows bisulfite sequence analysis of a cell line containing the High CpG / Low G + C construct in Dnmt 3a/b −/− cells, presented as in panel (B ).
There is no existing law that compels a researcher, at least in North America, to obtain formal consent from a biological relative prior to the publication of genome sequence data of a cell line, a reality that was rarely noted in the public response to the HeLa story.
"We apologized for any distress experienced by the family as a result of our recent publication of the genome sequence of a HeLa cell line.
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