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japonica (we did not use the ratio calculated for O. sativa var. indica sequence because we consider the map-based O. sativa var. japonica sequence more reliable) and that the Atlantys element and LTR sizes were 13,000 and 1,500 bp, respectively.
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More information could be obtained by deeper transcriptomic sequencing (more mapped sequences and more reliable SNP calling), use of longer sequences (increasing the possibility to find a parent relative SNP) or genomic DNA sequencing (where the intron sequencing, being more diverge region, could increase the number of parent relative SNPs).
Because gyrB sequence divergence is greater than that of 16S rDNA, phylogenetic analysis based on gyrB sequence (allowing more reliable identification of members of the genus Aeromonas [ 15]) identified isolate A37 as A. punctata subsp. punctata and isolate A39 as A. media.
ITS and partial EF1α gene sequencing are more reliable than partial β-actin and 28S rRNA gene sequencing for identification of the Lichtheimia species.
ITS and partial EF1α gene sequencing are more reliable than partial β-actin gene and partial 28S rRNA gene sequencing for identification of the Lichtheimia species.
Reading pitches and predicting sequences becomes more reliable with time.
The better the quality of the trimmed sequences, the more reliable the final assembly.
Careful analysis of the database sequences produced more reliable rice GH1 gene structure and protein product predictions.
Similar associations between mapping and sequence data may be established using marker and locus names, provided that marker nomenclature can be resolved Sequence-based connections between mapping data sets, integrated maps, and genomic sequence may be more reliable and are more amenable to automation than attempts to connect sources using names and other information.
Perhaps more importantly, DNA sequence markers are more reliable and discriminatory than AFLP genotypes.
However, it remains controversial whether high species sampling or high sequence coverage yields more reliable phylogenetic trees [ 83- 87].
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