In the absence of a matched germline DNA sequence, the misinterpretation of an inherited variant for a somatic mutation could potentially prevent a patient from getting appropriate genetic counseling.
Additional sampling is necessary to determine if this placement outside the Erectae is accurate or due to misidentification of the collections we sequenced or misinterpretation of the anatomical and morphological features.
These inherent features of DNA extracted from FFPE tissue can lead to assay failure, errors in amplification and sequencing, and subsequent misinterpretation of results since most assays are optimised to work with high quality and sufficient quantities of DNA isolated from fresh tissue.
On the other hand, computational analysis of sequence can prevent potential misinterpretation of experimental data.
For vehicles approaching in the opposite direction, clusters are continuously obtained throughout the sequence, sometimes resulting in misinterpretations of the bridge over the road.
Another type of sequencing error is the misinterpretation of one nucleotide instead of another.
Based on this data, we concluded that the presence of 25% of mutated template can lead to misinterpretation of sequencing results and may suggest that 25% of mutated template could be difficult to detect during sequencing of DNA.
To evaluate the robustness of the Likelihood Ratio Tests (LRTs), simulations have been performed that show that type 1 error rates can be up to 90% with relatively high rates of recombination in protein coding sequences resulting in the misinterpretation of recombination as positive selection [ 13].
This may have led artificial mismatches in exon-intron structure between the predicted CDS and the FL-cDNA sequences, resulting in misinterpretations of the alternative forms of the loci.
Recently, the use of sequencing was showed to yield misinterpretation leading to lack of response to anti-EGFR antibodies in mCRC bearing small KRAS-mutated DNA content [ 21, 22].
