From these SNPs, 105 SNP markers, six cleaved amplified polymorphic sequence markers (prefixed aa##), and one sequence tagged site marker (STS1) were developed (Additional file 1).
The strategy employed targets unique species-specific sequence markers in the second internal transcribed spacer (ITS-2) region of ribosomal DNA of the nematodes and utilises individual larval lysates as reaction templates.
The 434 rice accessions were also screened with two CAPS/dCAPS (derived cleaved amplified polymorphic sequence) markers Pi2SNP and Pi9SNP which were developed based on SNPs within the coding region of Pi2 and Pi9, respectively (Hua et al. 2015).
Variations in Alu-SNPs among the HapMap populations of northern and western European ancestry (CEU), Han Chinese from Beijing (CHB), Japanese from Tokyo (JPT), and Yoruba from Ibadan, Nigeria (YRI) indicate that the Alu-SNPs provide useful sequence markers, in addition to the Alu-insertion polymorphisms themselves, for the delineation of human genome evolution.
Perhaps more importantly, DNA sequence markers are more reliable and discriminatory than AFLP genotypes.
This study has shown that mitochondrial sequence markers can be useful tools for the identification of European marine fishes.
The present study showed that the investigated mitochondrial sequence markers perform differently in DNA barcoding and microarray analyses for the identification of fish species.
In this study, we investigate natural hybridization in Bruguiera over a wide geographical area in the IWP region using ISSR together with sequence markers.
All three mitochondrial sequence markers were useful for the identification of the 50 target species (Fig. 3, Fig. 4, and Fig. 5).
In addition, chloroplast and nuclear sequence markers provide an alternative means in elucidating the direction of hybridization and introgression at the species level.
An important element of phylogenetic analysis in a widely distributed marine genus such as Chthamalus is the ability to expand on the traditional sequence markers that have been used for biogeographic or phylogenetic study.
