Exact(6)
Despite these different FP tandems and dissimilarities within the amino acid sequence linking the VSD to the FPs, the amplitude and kinetics of voltage dependent fluorescence changes were surprisingly similar.
These data suggest that Stu2p function is compromised when the sequence linking two TOG domains becomes too short.
After restriction enzyme digestion and sequence linking, qPCR was performed using the forward and reverse primers (that is, F-primer and R-primer shown in the figure).
The fusion protein contains a total of 535 amino acid residues comprising Neu5Ac aldolase, the two tags, and the sequence linking GST and Neu5Ac aldolase.
Both shuffled linkers gave rescue activity nearly indistinguishable from the wild-type linker, consistent with the robust rescue activity of alternatively linked functional TOGs in dimeric Stu2p TOG1-TOG2(R519and and Stu2p TOG1 R200A -TOG2). Thus, the sequence linking the two functional TOG domains tolerates significant variation.
A good model must likewise be consistent with the lack of detectable positive cooperativity stabilizing the TOG1-TOG2:(αβ-tubulin 2 complex, and should not require different TOG domains or a non-spacer-like role for the sequence linking the two TOG domains.
Similar(54)
In another sequence linked to the falling van, Joseph Gordon-Levitt has a tumbling, weightless fight with a thug in a spinning hotel corridor.
Overall, the information suggested that lack of A-YHV virulence was associated with the NS gene sequence linked to ORF1b rather than the deletion in ORF3.
However, in the heterogenous format the recognition of target sequence occurs on a specific probe sequence linked to a solid support.
In addition, since this sequence links several different movements together (with one breath per movement), you are actually increasing energy flow and improving your cardiovascular system.
To examine the possibility that the DSE mutation reduces mRNA levels, we cloned N 3' UTR and downstream sequence, with and without the DSE mutation, after the GFP coding sequence linked to the actin 5C promoter.
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