Sentence examples for sequence limitation from inspiring English sources
It is thus desirable to use an expression system without this sequence limitation.
The observed protein sequence limitations can primarily be attributed to genetic codon degeneracy and signal peptidase cleavage preferences.
Therefore, it is important that annotation analyses run efficiently without excessive resource demands or sequence limitations.
The nucleotide and amino acid sequence limitations that cause small proteins to be challenging to identify using homology screens also lead to difficulties in construction of an evolutionary phylogeny.
While there are target sequence limitations created by the requirement of PAM before the target sequence, research into overcoming the initial limitations is showing that by using Cas9 orthologs with alternative PAM sequences [ 24– 26] and distinct sgRNA recognition sequences [ 27] there may be no practical limit to the targetable sites in the genome.
In addition to these sequencing limitations, a challenge in relation to the use of WES in case control studies for complex diseases will be to identify rare causal variants in unrelated cases.
In addition, we present a simple and robust solution for overcoming sequencing limitations commonly encountered with shRNA vectors.
Neither repositioning the sequencing primer, nor the addition of molecular disruption agents to the reaction were able to overcome sequencing limitations (data not shown).
The k-mers are aligned and shifted before calculating the frequencies as described in (Schmieder et al., 2010) to account for sequencing limitations.
Assuming the current Roche/454 Titanium sequencing limitations, a 12 Mbp region could be re-sequenced with a full plate of linear reads and a half plate of paired-end reads, yielding 15L-5P coverafterftereadad pre-processing.
As a result of these EST sequencing limitations mentioned above, the number of identified SNPs is relatively low in many species with validation rates usually between 50 and 85% [ 12].
