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Lucy program [ 50] was used to remove the vector sequences and short sequence less than 200 bp.
The new reactions produced a double sequence less than 80 bases from the end of the PCR fragment (P0 site).
A domain bigram, consistent with the definition of domain combination in SUPERFAMILY, is defined as an adjacent domain pair with gap sequence less than 30 amino acids.
We removed any simulated transcript sequence less than 200 nt long, leaving us with a total of 99 simulated fusions, 100 simulated PTDs, and 100 simulated ITDs.
We next combined HSVs co-locating within a region of gene sequence less than the 51 base pair length of an individual sequence read to produce haplotypes.
Another important fact to stress is that IPknot can run quite fast even on a relatively long sequence less than one thousand bases.
Similar(48)
According to recommendations by Wicker et al.71, sequences less than 80 bp in length were excluded to avoid misclassification.
Thermal melting curve analysis of DNA duplexes on biomagnetite indicated that shorter repeat sequences (less than 9 repeats) were easily discriminated.
Short BLAST72 hit fragments less than 500 bp, and putative short repeat sequences less than 1250 bp, which showed more than two BLAST hit blocks within the 700 kb region, were filtered using a custom Perl script.
Consensus sequences less than 300 bp were removed, as were sequences with BLASTn homology to plant sequences.
These previous works were all based on limited sets of sequences (less than 350 proteins) compared by multiple sequence alignments.
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