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Due to the initial choice of a medium-size DNA library which could be completely covered by our 454-sequencing effort, in this study we were not able to fully sequence large introns or to recover regulatory gene elements.
The ability to cheaply sequence large volumes of DNA has already led to the discovery of new disease genes and improved our understanding of evolution and human history.
The ability to routinely sequence large populations of single cells will enable the de-convolution of genetic heterogeneity in diverse cell populations.
This experiment has shown that it is possible to sequence large complex plant genomes such as pepper using the 10x Chromium technology and customized, open-source Supernova assembler.
It also makes it possible to sequence large collections of individual genomes to very high quality, something not tractable with more expensive long-read sequencing.
In earlier sites this is likely to be a small, thin, portable slab of stone used with a small pebble handstone, while later in the sequence, large, basin-shaped milling stones are more characteristic.
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Such studies would also help researchers to unpick the effects of combinations of genetic variants, a consideration that is likely to become more important as clinicians sequence larger sets of a tumour's genes.
However, it is crucial to have reliable empirical results when making recommendations about which gene regions to sequence large-scale as standards [24].
Primer walking was used to sequence larger insert clones.
No sequence larger than 300 nucleotides was identified showing significant similarities to LGRs.
"These studies provide the first really well-delineated set of road maps". Chin and Velculescu hope that sequencing costs will soon drop low enough to allow them to combine the two techniques, sequencing large numbers of genes in many tumors.
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