Sentence examples similar to sequence is digested from inspiring English sources

The HC-ProR182K sequence was digested with Mfe I/Kpn I and ligated with the same enzyme-digested pBD-TuMV-GFP to generate pBD-TuMV-GK (Figure 2C).

Amplified SAM sequence was digested with Bam H I and Eco R I and cloned in to pPIC3.5 K vector.

Wild type molecules at the enzyme-specific sequence are digested, while mutant molecules are not.

The phosphoglycerate kinsase sequence was digested from pBC293 (Cohen Lab, Washington University) using EcoRI and BglII restriction enzymes.

Cell lines containing the wild-type pol ι sequence are digested by TaqαI to yield 39 and 49 base pair fragments.

The amplified MeCP2(1 308) sequence was digested with XcmI and XbaI and ligated, using T4 DNA ligase (NEB), into pcDNA3.1 human MeCP2A or B myc-tagged plasmids digested with the same enzymes.

In brief, the selected vector with correct DNA sequence was digested with Kpn I (New England Biolabs), blunted by T4 DNA polymerase (New England Biolabs) with dNTP, and digested with Bam HI (New England Biolabs).

The full-length TCR chain with BCG-specific CDR3 sequence was digested with KpnI and XhoI and cloned into pREP7 and pREP9 expression vectors with either hygromycin or neomycin resistance.

The amplified full-length RcPutA sequence was digested with NdeI and XhoI and then cloned into pET-28a.

The amplified HSP70 and MMP-II sequence were digested with appropriate restriction enzymes and cloned into parental pMV261H plasmid.

Binary vector p35S AtNPR1 EGFP - The pGEM-AtNPR1 containing AtNPR1 coding sequence was digested with NcoI and the fragment was ligated into the NcoI site of pE2113 as described above.