In this study we demonstrate that pooled sequencing is applicable for the analysis of sequence variations of strain collections with more than 10 individual isolates.
The data obtained demonstrate that whole genomic sequencing is applicable as a high-throughput tool to investigate genomic changes due to mutagenesis.
They were also able to recover many motifs from three mixed strains (E. coli, G. metallireducens, and C. salexigens), even when the motif sequences of the genomes of interest overlap substantially, suggesting that PacBio sequencing is applicable to metagenomics.
Since our algorithm was originally developed based on discoveries and studies concerning siRNA functionality, its high success rate in shRNA design suggests that most design concerns for siRNA sequences are applicable to shRNA design.
The main limitation of this approach is that, in order to guarantee the convergence, one has to assume a certain smallness of the eigenvalues of the matrix p K. It was shown, in particular, in [6] that the method based upon sequence (4) is applicable provided that r ( K ) < 1 γ 0 p, (11).
HER2 is an important clinically actionable target, indicating this type of targeted sequencing analysis, which has greater sensitivity than exome sequencing and is applicable to formalin-fixed paraffin-embedded tissue, may soon aid in the management of patients when planning primary or secondary treatment regimes.
Once users submit their jobs, ListeriaTree pipeline starts retrieving all sequences of the marker genes of the user-selected strains (as well as the user-submitted sequence if it is applicable) and store them into a temporary FASTA file for alignment using MAFFT (Multiple Alignment using Fast Fourier Transform) [ 44].
It encompasses all steps from sample preparation to sequence analysis and is applicable to baseline profiling or perturbation measurements.
However, with the advancement of next-generation sequencing technique, it is applicable to provide insight into cell wall biosynthesis in Miscanthus by de novo transcriptome analysis via RNA-seq.
Moreover, VFS is generalized to major sequencing platforms, and is applicable to both DNA- and RNA-Seq data.
