Sentence examples for sequence integrity from inspiring English sources

Restriction digestion, PCR amplification and/or sequencing were carried out to ensure sequence integrity and orientation.

The in-house vector presented here was constructed by combining commercial pUC18 and the pDONR-221 plasmid's ccdB gene encoding region; toxic gene system sequence integrity was verified by sequencing, corresponding to the expected size (659 bp).

The sequence integrity of all constructs described in this study was verified for sequence integrity by nucleotide sequencing.

All the plasmids containing CmADH genes were sequenced to verify sequence integrity (AuGCT, Beijing, China).

The examination of multiple PCR reactions for specific tissues enabled the confirmation of sequence integrity in the database.

Restriction digests and sequencing were used to confirm the sequence integrity of the resulting shuttle (pShuttle-CMV-HIV-Gag).

All described constructs were sequenced for integrity, and pure plasmid DNA was isolated by using a plasmid maxi kit (Qiagen).

The fragment was cloned into pGEM T-Easy (Promega Corporation, Madison WI) according to the manufacturer's instructions and sequenced for integrity.

Prior to sequencing, RNA integrity numbers were determined for poly(A) mRNA isolated from each of the four developmental stages using a Bioanalyzer 2100 (Agilent Technologies).

On 7 April, 7 days after the announcement of the outbreak and the posting of the gene sequences, the integrity of the transcribed RNA was demonstrated by agarose gel electrophoresis, and expression of influenza H7 HA in the transfected BHK cells was demonstrated by sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by Western blot analysis with an H7-specific antibody.

A detailed description of the sequenced samples RNA integrity numbers (RIN) and sequence alignment characteristics can be seen in Supplementary Table S4.